- Sand Gravel & Aggregate, Middleburg
- Sandwich Shop Restaurants, Middleburg
- Savings & Loan Associations, Middleburg
- Savings Banks, Middleburg
- Screen Printing, Middleburg
- Septic Tanks & Systems, Middleburg - Wholesale, Dealers & Suppliers
- Service Establishment Supplies & Equipment, Middleburg
- Sewer Construction Contractors, Middleburg - Sewer Line, Pipe Installation & Repair Services
- Shooting & Target Ranges, Middleburg
- Shopping & Shopping Services, Middleburg
- Siding Contractors, Middleburg - Vinyl Siding, Wood, Metal & Aluminum Siding Contractors - Installation & Repair
- Snacks & Desserts, Middleburg
- Social Services & Welfare, Middleburg
- Societies & Foundation Associations, Middleburg
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- Sororities, Middleburg
- Specialty Animal Services, Middleburg
- Specialty Stores, Middleburg
- Sporting Goods Stores, Middleburg
- Sprinkler Alarm Systems, Middleburg - Fire Sprinkler Systems
- Sprinkler System Contractors, Middleburg - Garden & Lawn Sprinkler System Installation & Repair
- State Government, Middleburg
- Stone & Rock Quarry, Middleburg - Gravel Pit, Shale, Granite, Limestone, Crushed Rock, etc
- Stone Setting Contractors, Middleburg
- Substance Abuse Clinics, Middleburg - Alcoholism, Addiction & Drug Abuse Treatment Centers
- Surveyor Services, Middleburg - Surveying Service
- Surveyors, Middleburg
- Swimming Pools, Middleburg
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